منابع مشابه
Epigenetic analysis of regulatory T cells using multiplex bisulfite sequencing
A subset of regulatory CD4+ T cells (Tregs) is characterized by the stable constitutive expression of the transcription factor FOXP3. Demethylation at a conserved region within intron 1 of FOXP3, the Tregspecific demethylated region (TSDR), is exclusive to this subset of Tregs: other immune cells that do not express FOXP3 or express the transcription factor transiently, such as activated effect...
متن کاملAmplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging
DNA methylation plays a key role in epigenetic regulation of eukaryotic genomes. Hence the genome-wide distribution of 5-methylcytosine, or the methylome, has been attracting intense attention. In recent years, whole-genome bisulfite sequencing (WGBS) has enabled methylome analysis at single-base resolution. However, WGBS typically requires microgram quantities of DNA as well as global PCR ampl...
متن کاملWhole-Genome Bisulfite Sequencing
Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...
متن کاملWhole-Genome Bisulfite Sequencing
Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...
متن کاملNGS-based deep bisulfite sequencing
We have developed an NGS-based deep bisulfite sequencing protocol for the DNA methylation analysis of genomes. This approach allows the rapid and efficient construction of NGS-ready libraries with a large number of PCR products that have been individually amplified from bisulfite-converted DNA. This approach also employs a bioinformatics strategy to sort the raw sequence reads generated from NG...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2019
ISSN: 1548-7091,1548-7105
DOI: 10.1038/s41592-019-0387-x